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Porcine FVIII Nijmegen Bethesda assay-994

Test info

Test name:

Porcine FVIII Nijmegen Bethesda assay

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Useful for:

To quantitate the titer of FVIII-specific inhibitors to porcine FVIII concentrate

Specimen

Specimen type:

Sodium citrate (Na cit) plasma

Collection container:

Lt blue Sodium citrate (NaCit) - 2.7mL

Volume:

1.5 mL

Minimum volume:

0.75 mL
Note: this volume does not allow for repeat testing

Collection instructions:

Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.
Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio.
- Coag - Tube fill guidelines
Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Processing instructions:

Spin
Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as Na cit plasma
Freeze

Transport container:

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Transport and stability:

Frozen (strict)

Reason for rejection:

Thawed sample
Wrong sample type

Performance

Performing lab:

Esoterix Coagulation Lab (301608) via LabCorp (504474): R-NX

Days set up:

Weekly (varies)

TAT:

8 days

Method:

One-stage clotting, Nijmegen-modified Bethesda assay

Clinical and Interpretive info

Reference ranges:

< 0.6 BU/mL

Billing

CPT codes:

85335

LOINC code:

Result 3204-5

Tracking

Date created:

05/24/2019

Revised date:

12/18/2023

Review date:

12/18/2023