The HBV genotype assay classifies the virus present in the sample into one of nine recognized genotypes (A-I) via sequence analysis of the DNA polymerase gene. Disease progression and response to therapy may be influenced by genotype. In addition, the HBV genotype assay identifies and reports sequence polymorphisms in the basal core promotor (BCP) and coding regions of the HBV precore gene that result in either decreased levels, or complete absence, of HBeAg expression in chronically infected patients.

This assay may not be successful when the HBV viral load is <500 IU/mL.

Gold serum separator (SST) tube

Immediatley following collection, mix sample by inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within 6 hours of collection
3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
4. Freeze

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Lavender (EDTA), 4mL

Yellow ACD (A or B)

1. Spin within 6 hours of collection
2. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as plasma
3. Freeze

Frozen (preferred) - 14 days

Refrigerated - NO

Ambient - NO

• Quantity not sufficient for analysis
• Heparin/SPS plasma (wrong anticoagulant)
• Gross hemolysis
• Specimen not frozen

Polymerase chain reaction (PCR) amplification and DNA sequencing

An interpretive report will be provided