• Antibody titer will include antibody identification and appropriate red cell antigen typing(s) as required
• Each serum technique/panel needed will be charged separately.
• Other special testing may be required for significant antibody identification and will be performed at an additional charge

Two (2) Lavender (EDTA), 4mL tubes

In certain circumstances, additional blood may be requested to complete testing. If the patient has a known antibody, send 2 - 3 4mL EDTA whole blood tubes for testing.

Lavender:

Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting.

Labelling instructions:
Excellian sites:
Tube must be labeled with the patients first and last name and Excellian MRN, date and time of collection and phlebotomist’s A#

Non-Excellian sites:
Tube must be labeled with the patient's first and last name and date of birth, date and time of collection and phlebotomist’s initials.

Submit tubes unspun

Two (2) Lavender (EDTA), 4 mL tubes

Refrigerated - 3 days (96 hours)

• Improper label (unlabeled or mislabeled)
• Hemolysis

Hemagglutination

Negative

If postive:

• The specificity of the maternal alloantibody will be stated. The titer result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.
• If the alloantibody identified is not relevant in hemolytic desease of the newborn, the titer will be canceled.
• Autoantibodies are not titered.

Some maternal IgG alloantibodies to red blood cell antigens will cross the placenta and cause hemolysis of antigen-positive fetal red blood cells. The resulting fetal anemia and hyperbilirubinemia can be harmful or possibly fatal to the fetus/newborn. Titers are performed to assess the potential strength of alloantibody.