Slide holder - 5 specimen

Screw cap plastic container (sterile)

Urine Cytospin:

1. Within 4 hours of collection, centrifuge 12 cc freshly voided urine as for routine urinalysis
2. Decant
3. Check the sediment.
   1. If there are more than 25 WBC/HPF, make two cytospin smears from the unspun, well-mixed urine.
   2. If there are less than 25 WBC/HPF, make two cytospin smears from the spun sediment
4. Label slides; Include patient name, date, sample # and specimen type
5. Refer to Cytospin Operating Procedure. Always add 1 drop of 22-30% Specific Albumin to the cuvette before spinning.

If no Cytospin is available, transfer 10mL of urine sample to a Urine aliquot transport vial/tube - 10mL (AHL). The urine sample must be delivered to the AHL Central Laboratory within 4 hours of collection.

Sputum:

Screw cap plastic container (sterile)

Urine:  

Two (2) cytospin slides in slide holder

Urine aliquot transport vial/tube - 10mL (AHL)

Slides: Ambient

Sputum: Ambient - transport sputum as soon as possible

Urine: time sensitive

Refrigerated - 4 hrs

• Improper label (unlabeled or mislabeled)
• Delay in transport
• Improper storage/transport temperature
• Slides broken beyond repair

Microscopy

Few or no eosinophils