Residual T - Acute Lymphoblastic Leukemia-12420

Test info

  
Residual T - Acute Lymphoblastic Leukemia
  
12420
  
LAB12420
  
Flow
Flow Cytometry
Immunophenotyping
Acute Leukemia
Lymphoblast
Precursor
Blast
T-ALL
T cell leukemia
  
Tubes:
T1
T2
T3*
*Varies according to diagnostic phenotype

Markers may include:
CD1a
CD2
CD3
CD3(cytoplasmic)
CD4
CD5
CD7
CD8
CD10
CD16
CD19
CD34
CD45
CD56
CD57
HLA-DR
TCR alpha beta
TCR gamma delta
TdT(nuclear)
TRBC1
*Varies according to diagnostic phenotype
  

Monitor residual T-ALL blasts based on pre-treatment phenotype

  

Pathology orders only

Specimen

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Cerebrospinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B+ fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container

Performance

  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

Clinical and Interpretive info

  

Tubes and markers:

Tubes:

T1, T2, T3*

Markers*:

CD1a, CD2, CD3, CD3(cyto), CD4, CD5, CD7, CD8, CD10, CD16, CD19, CD34, CD45, CD56, CD57, HLA-DR, TdT(n), TCRαβ, TCRγδ, TRBC1

*Varies according to diagnostic phenotype

Billing

  
88184
88185 x 19*
* Varies according to diagnostic phenotype

Tracking

  
08/05/2021
  
03/14/2022
  
01/09/2024