RUNX/RUNX1 or t(8;21) is a recurring abnormality in AML.  Current NCCN guidelines for reporting prognostic cytogenetic indicators are utilized.

This test can be ordered individually or as part of the AML FISH Panel. See the panel test for more details

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

1. Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
2. Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Transfer aspirate from syringe to transport container. 

Submit entire specimen unspun.

Sterile vial/tube

or

Dk green Sodium heparin (Na hep), no gel

Bone marrow core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep), no gel

Whole blood:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting.

Submit entire specimen unspun

Bone marrow core:

Sterile vial/tube

Whole blood:

Dk green Sodium heparin (Na hep), no gel

AHL Cytogenetics Oncology Request

Ambient

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Delay in transport
• Improper storage
• Leaking container
• Slides broken beyond repair

Fluorescence in situ hybridization (FISH)

Normal or Abnormal with interpretation

Medical necessity