Detection of the presence of cold agglutinins in patients with suspected cold agglutinin disease.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Transport blood immediately to laboratory
2. Allow to clot, incubated at 37°C, for a minimum of 30 minutes
3. Spin to separate serum from cells. Do NOT refrigerate prior to separation of serum from red cells.
   • If there is not a 37°C incubator, allow to clot at room temperature and separate serum from cells immediately after clotting.

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

1. Transport blood immediately to laboratory
2. Allow to clot, incubated at 37°C, for a minimum of 30 minutes
3. Spin.  Do NOT refrigerate prior to separation of serum from red cells.
   • If there is not a 37°C incubator, allow to clot at room temperature and separate serum from cells immediately after clotting.
4. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum

Transfer vial/tube with cap - 12mL (LabCorp)

Refrigerated

Do NOT refrigerate prior to separation of serum from red cells.

• Refrigeration of the specimen before separating serum from cells

Hemagglutination

Negative: < 1:32

This test is not specific for Mycoplasma pneumoniae. For diagnosis of Mycoplasma pneumonia infections, see Mycoplasma pneumoniae Antibodies, IgG, IgM (13538).