• All IHC stains will include a positive control tissue

• Beta amyloid is used to identify amyloid angiopathy, and is more sensitive than congo red stains
• This antibody can also identify Alzheimer's neurofibrillary tangles
• This antibody will not identify the other types of amyloid (use kappa and lambda to identify primary amyloidosis/plasma cell dyscrasias; we don't have antibodies to the SAA (serum amyloid associated protein) or beta 2 microglobulin protein types of amyloid

Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue block

Tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Beta amyloid is an extracellular filamentous protein deposit found in the brain, and is the major protein of amyloid cores, neuritic plaques, and neurofibrillary tangles
• This protein is found in Alzheimer's disease, and other rare types of dementia
• Beta amyloid is also found in amyloid angiopathy, which is a leading cause of spontaneous intracerebral hemorrhage in the elderly

Staining patterns

• Cytoplasmic staining

References

1. Ishihara T et al: The significance of cerebrovascular amyloid in the aetiology of superficial (lobar) cerebral hemorrhage and its incidence in the elderly population. J Pathol 1991; 165:229-234.
2. Vinters HV et al: Immunohistochemical study of cerebral amyloid angiopathy. III: Widespread Alzheimer A4 peptide in cerebral micro-vessel walls co-localizes with gamma trace in patients with leukoencephalopathy. Ann Neurol 1990; 28: 34-42.