Data generated in the genotyping assay is subsequently analyzed for mutations in the reverse transcriptase gene of HBV associated with resistance to therapeutic agents, and if identified an interpretation of their clinical significance provided.

This assay may not be successful when the HBV viral load is <500 IU/mL

Lavender (EDTA), 4mL

Immediatley following collection, mix sample by inverting 5 times

1. Spin within 6 hours of collection
2. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as plasma
3. Freeze

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Gold serum separator (SST) tube

or

Yellow ACD (A or B)

Immediatley following collection, mix sample by inverting 8-10 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within 6 hours of collection
3. Transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as plasma
4. Freeze

Frozen (preferred) – 14 days

Refrigerated- NO

Ambient - NO

• Quantity not sufficient for analysis
• Heparin/SPS plasma (wrong anticoagulant)
• Gross hemolysis
• Specimen not frozen

Polymerase chain reaction (PCR) amplification and DNA sequencing

An interpretive report will be provided