Data generated in the genotyping assay is subsequently analyzed for mutations in the reverse transcriptase gene of HBV associated with resistance to therapeutic agents, and if identified an interpretation of their clinical significance provided.

This assay may not be successful when the HBV viral load is <500 IU/mL

Lavender (EDTA), 4mL

Immediatley following collection, mix sample by inverting 5 times

• Centrifuge sample within six hours of collection

• Transfer plasma or serum to a Labcorp transport vial and freeze

Labcorp freeze vial (purple cap)

Gold serum separator (SST) tube

or

Yellow ACD (A or B)

• Immediatley following collection, mix sample by inverting 5 times

• Allow SST to clot for 30 minutes before spinning

• Centrifuge sample within six hours of collection

• Transfer plasma or serum to a Labcorp transport vial and freeze

Frozen (preferred) – 14 days

Refrigerated- not accepted

Ambient - not accepted

• Quantity not sufficient for analysis
• Heparin/SPS plasma (wrong anticoagulant)
• Gross hemolysis
• Specimen not frozen

Polymerase chain reaction (PCR) amplification and DNA sequencing

An interpretive report will be provided