All IHC stains will include a positive control tissue

• This antibody is useful in identifying hyperplastic breast epithelium (CK5/6 positive)
• Neoplastic breast proliferations (ADH, DCIS) are typically CK5/6 negative
• CK5/6 also is a marker of breast myoepithelial cells
• This antibody will also stain squamous epithelium of normal skin
• CK5/6 is useful in separating mesotheliomas from pulmonary adenocarcinomas; it will stain mesothelial cells and mesotheliomas, but it is typically negative in adenocarcinomas

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CK5/6 is an antibody cocktail that reacts with high-molecular weight keratins (56,58 kD)

Staining pattern

• Cytoplasmic

References

1. Cury PM et al; Mod Pathol 2000;13:107-12.
2. Otterback F et al: Histopathology 2000;37:232-40.