• All IHC stains will include a positive control tissue

• Useful as a general screening marker for germ cell tumors and is positive in most
• Classic seminomas/dysgerminomas are almost all PLAP positive
• Spermatocytic seminomas are usually negative for PLAP
• Embryonal carcinomas are usually (95%) positive for PLAP
• Teratomas may or may not stain positively for PLAP
• Endodermal sinus tumors are usually positive for PLAP
• Choriocarcinomas typically are positive for PLAP
• Intratubular germ cell neoplasia usually stains positively for PLAP
• Various somatic carcinomas, including those of breast, lung, GI tract, ovary, prostate and bladder origin, have been reported to be positive for PLAP on occasion
• PLAP positivity has also been reported in partial and complete hydatiform moles

Note: There is cross-reactivity of this antibody with intestinal alkaline phosphatase.
Staining of striated muscle with PLAP has been observed and reported.

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Anti-placental alkaline phosphatase labels trophoblasts in placental tissue

Staining pattern

• Cytoplasmic and apical based staining

References

1. Ulbright TM: Germ Cell Neoplasms of the Testis; American Journal of Surgical Pathology; 17(11):1075-1091, 1993.