Hepatitis D virus (HDV) is an incomplete RNA virus that requires the helper function of Hepatitis B virus (HBV) envelope proteins (HBSAg) to replicate. The presence of HDV antibodies indicates exposure to HDV, but does not distinguish acute or chronic HDV infection. Typically the disappearance of HDV antibodies is indicative of resolution of infection.

Gold serum separator (SST) tube

Immediately following collection, mix sample by gently inverting 5 times

1. Allow sample to clot
2. Spin within two (2) hours of sample collection
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
4. Freeze

Transfer vial/tube with cap - 12mL (LabCorp)

Ambient (preferred) – 14 days

Refrigerated – 14 days

Frozen – 14 days

Freeze/thaw cycles - stable x3

• Gross hemolysis or lipemia
• Visible microbial contamination
• Heat-inactivated samples

The HDV total antibody ELISA is a competitive immunoassay.

Anti-HDV antibodies, if present, compete with HRP-conjugated polyclonal anti-HDV antibodies for recombinant HDV antigen adsorbed to the microtiter plate.

Negative