HBc total antibody measures both IgG & IgM but when reactive, does not distinguish between the two. If acute infection is suspected, order an Anti-HBc IgM

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

Lt green plasma separator (PST)

Lavender (EDTA), 4mL

Lt green/Lavender:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Red:

1. Allow sample to clot
2. Spin within two (2) hours of sample collection
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL), labelled as serum

Lt green PST:

Spin within two (2) hours of sample collection

Lavender:

1. Spin within two (2) hours of sample collection
2. Transfer plasma to a False bottom plasma/serum transport vial/tube (AHL), labelled as the appropriate plasma type

Plasma separator (Lt green PST)

False bottom plasma/serum transport vial/tube (AHL)

Refrigerated (preferred) - 14 days

Ambient - 7 days

Frozen - 3 months

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

Electrochemiluminescence immunoassay (ECLIA)

Non-reactive

Medical necessity