• All IHC stains will include a positive control tissue

• Arginase-1 reactivity is seen in the vast majority of well-differentiated hepatocellular carcinomas (HCC), but may be absent in poorly differentiated HCCs
• This antibody is more sensitive than HepPar-1 in identifying HCC²
• This antibody is very useful with Glypican 3 in identifying scirrhous HCC (more useful than HepPar-1)
• One study showed rare non-hepatic tumors with Arginase reactivity (cholangiocarcinoma and prostatic adenocarcinoma)²

References

1. Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
2. Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
3. Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

Tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Arginase-1 is a urea cycle metalloenzyme that is expressed in liver
• This antibody is a sensitive and specific marker that can be used to confirm hepatocellular differentiation and exclude other possible primaries

 Staining patterns

• Strong diffuse cytoplasmic staining; patchy nuclear staining can also be seen

References

1. Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
2. Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
3. Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.