• All IHC stains will include a positive control tissue

• Most inflammatory HAs show strong SAA staining, which can be patchy or diffuse (>90%)
• SAA is negative in most (83%) of FNH cases (rare cases show patchy or diffuse staining)

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

Tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• This antibody labels amyloid A (AA) in tissues and can be used to classify AA-amyloidosis.
• This antibody does not react with other amyloid types, such as Alzheimer’s amyloid plaques
• SAA can be used in a panel with glutamine synthetase and beta catenin to help differentiate between hepatic adenomas (HA) and focal nodular hyperplasia (FNH)

Staining patterns

• Strong cytoplasmic staining

References

1. Linke RP: Monoclonal antibodies against amyloid fibril protein AA. Production, specificity and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 1984;32:322-8.
2. Shafizadeh N et al: Diagnosis of well-differentiated hepatocellular lesions: role of immunohistochemistry and other ancillary techniques. Adv Anat Pathol 2011;18:438-445).
3. Joseph NM et al: Diagnostic utility and limitations of glutamine synthetase and serum amyloid-associated protein immunohistochemistry in the distinction of focal nodular hyperplasia and inflammatory hepatocellular adenoma. Modern Pathology (2014) 27, 62–72; doi:10.1038/modpathol.2013.114.