• Screening performed at 1:10 dilution
• Titers performed - 1:20, 40, 80, 160, 320 and 640

Antibodies to native, double-stranded DNA (anti-nDNA) are found in up to 60% of patients with SLE, in almost all cases of lupus nephritis, and can be found in rheumatic diseases.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube - 5 mL

Red/grey serum separator (SST) vial/tube, 10 mL

1. Allow sample to clot
2. Spin
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL), labelled as serum, within two (2) hours of sample collection

False bottom plasma/serum transport vial/tube (AHL)

Red/grey serum separator (SST) vial/tube, 10 mL

Refrigerated (preferred) - 7 days
Frozen (OK)

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

DNA Double-Stranded (dsDNA) Antibodies by Crithidia luciliae IFA, IgG, serum

Negative

The titer of the anti-nDNA may decrease with successful therapy and increases in acute recurrence of the disease.

Screening performed at 1:10 dilution. Titers performed - 1:20, 40, 80, 160, 320 and 640.