Rearrangements of MAFB/IGH are recurring abnormalities in plasma cell dyscrasia disorders including Plasma Cell Myeloma.  According to current mSMART 3.0 myeloma guidelines, rearrangement of MAFB/IGH is classified as a high-risk cytogenetics category. 

This test can be ordered individually or as part of a Plasma Cell Dyscrasia FISH Panel (See the individual panel test for more details).

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

1. Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
2. Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Transfer aspirate from syringe to transport container.

Submit entire specimen unspun.  Specimen will be CD138 enriched for plasma cell isolation.

Sterile vial/tube

or

Dk green Sodium heparin (Na hep), no gel

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

or 

Whole blood:

Dk green Sodium heparin (Na hep), no gel

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

Submit entire specimen unspun

Sterile tube/cup

or

Dk green Sodium heparin (Na hepl), no gel

AHL Cytogenetics Oncology Request

Ambient

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Delay in transport
• Improper storage
• Leaking container
• Slides broken beyond repair

Fluorescence in situ hybridization (FISH)

Normal or Abnormal with interpretation

Medical necessity