Direct antiglobulin test-6085

Test info

  
Direct antiglobulin test
  
6085
  
86880.3
  
DAT
  
Coombs, direct
DAT
  
  • Extended DATs using IgG and C-3 specific antisera may be performed at additional charge if Coombs, Direct is positive
    • An RBC elution and any necessary antibody ID may be performed at additional charge if indicated
  
Demonstrating in vivo coating of red blood cells with IgG or the complement component C3d in the following settings:
  • Autoimmune heomlytic anemia
  • Hemolytic or delayed transfusion reactions
  • Drug induced hemolytic anemia

Specimen

  
EDTA whole blood
  

Lavender (EDTA), 4mL

  
4.0 mL
  
Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting
 
Labelling instructions:

Excellian sites:
Tube must be labeled with the patients first and last name and Excellian MRN, date and time of collection and phlebotomist’s A#

Non-Excellian sites:
Tube must be labeled with the patient's first and last name and date of birth, date and time of collection and phlebotomist’s initials.
  

Lavender (EDTA), 4mL

  

Refrigerate - 3 days

Sample should be obtained no more than 24 hours prior to testing but may be used up to 3 days post collection. Testing of older specimens may show decreased antibody reactivity.

  
  • Improper label (unlabeled or mislabeled)
  • Hemolysis

Performance

  
AHL - Blood Bank: B
  
Daily
  
1 day
  

Column-agglutination, agglutination

Clinical and Interpretive info

  

Negative

  

The direct antiglobulin test (DAT) is a screening test to determine if red cells have been coated in vivo with immunoglobulin, complement, or both.

Billing

  
86880

Tracking

  
11/30/2001
  
12/27/2023
  
12/21/2023