28s rDNA Broad Range Fungal PCR-994

Test info

  
28s rDNA Broad Range Fungal PCR
  
994
  
LAB994
  
MSO
  
23s
26s
28s
28-s
28s sequencing
FNA
Fungal sequencing

Specimen

  
Fresh frozen tissue (bone, bone marrow, fine needle aspirate, lung, lymph, muscle or spleen)
  

Sterile vial/container

  
1 cm x 1cm
  
None
Senstivity is decreased with lower volumes of tissue or fluid
  

Preferred - Fluid or tissue for broad range bacterial DNA testing should be put in a sterile container by the provider at the time of the procedure.  This ensures that the specimen won’t be opened until it reaches the testing laboratory and prevents the opportunity for contamination.

Acceptable - OK to add on to an open container.  Order must be placed within 7 days of collection.  Freeze immediately upon receipt of 16s order.

16s (broad range bacterial PCR) may be ordered on this same specimen, and will be performed at an additional charge.  Order LAB12804

Contact the laboratory medical director through Client Services if there are questions.

  

Submit entire specimen, Frozen.

  

Sterile vial/container

  
Abscess
Aspirate/fluid (abdominal, amniotic, ascitic, BAL, bile, CSF, cyst fluid, paracentesis, pericardial, pleural, synovial, thoracentesis or vitreous)
  

Sterile container

  
2.0 mL
There is no minimum volume, however, sensitivity is decreased with lower volumes
  

For all sites other than Phillips Eye:

For Phillips Eye (PEI) only:

  • U of Washington request form
    • The following items are required:
      • Patient name
      • Patient DOB
      • Ordering physician
      • Ordering physician phone number
      • Specimen source
      • Date and time collected
      • ICD/Diagnosis
  

Frozen (preferred) - 21 days

Refrigerated - 48 hours

Ambient /room temp - 2 hours

  
  • Diluted fluid sample, including eye/vitreous 
  • Specimens collected on swabs
  • Tissues free-floating in formalin
  • Samples containing SPS, citrate, or heparin
  • Blood/serum specimens

Performance

  
U of Washington via Hennepin County Medical Center/HCMC (LAB0001837): R-NX; PEA only send to U of Washington
  
6 days
  

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Clinical and Interpretive info

  

No fungal 28s rDNA detected

Billing

  
87801

Tracking

  
12/29/2019
  
05/20/2024
  
03/17/2022