Direct Venipuncture collection:
- Using a 21 gauge needle or larger (butterfly is acceptable), collect tubes in the following order:
- One (1) clear top Vacutainer tube - partial fill 3 mL draw
- Three (3) Lt blue Greiner tubes; these are partial fill 2 mL draw tubes. Fill exactly to the black arrow on the tube; do not over or under fill
- One (1) lavender top tube
- Gently invert each tube 5 times
Additional tubes may be collected after these tubes have been collected
Line draw collection
- Discard the first 5 mL of blood from the line
- Ensure the catheter is free of clots
- Immediately transfer blood in the following order:
- One (1) clear top vacutainer tube - partial fill 3 mL draw
- Three (3) lt blue Greiner tubes; these are partial fill 2 mL draw tubes. Fill exactly to the black arrow on the tube; do not over or under fill
- One (1) lavender top tube
- Gently invert each tube 5 times
Additional tubes may be collected after these tubes have been collected.