Establish the presence and possible etiologic role of Helicobacter pylori in cases of chronic gastric ulcer, gastritis, duodenal ulcer, dyspepsia, etc

Stool transport media/vial - Culture and sensitivity (C&S)

• Anaerobic transport
• aerobic/anaerobic bacterial swab transport system
• CLO vial
• Urea agar medium

Ambient - 72 hours

Culture

No Helicobacter pylori isolated

For optimal recovery of Helicobacter pylori, it is strongly recommended that culture be performed within three hours of specimen collection. Helicobacter pylori has been implicated as a factor associated with chronic gastritis. The clinical significance of the organism in regard to gastric or duodenal ulcers, dyspepsia, and gastric carcinoma remains unclear.1 Other factors, such as pepsin, nonsteroidal anti-inflammatory agents (NSAIDs), aspirin, ischemia, stress, alcohol, and bile salts as well as H pylori, all may have a role in altering the material mucosal barrier. The relative etiologic role of H pylori remains a subject of speculation. H pylori is hypothesized to secrete products capable of degrading gastric mucin. The organism may be seen in Gram stains on imprints from tissue biopsies. In one study, imprint Gram stains performed by imprint technique on biopsies from both the antrum and fundus yielded positives in 100% of 32 culture-positive cases.