• All IHC stains will include a positive control tissue

• In paraffin tissue, the antibody specifically and quantitatively labels toxo trophozoites
• Identification of toxo is especially important in the immunocompromised host
• Identification of toxo in the placenta correlates with congenital toxoplasmosis and can be associated with mental retardation and blindness

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Toxoplasma is a sporozoan that lives as intracellular parasites in various tissues and organs of vertebrate animals and completes its life cycle in a single host
• This protozoan can be transmitted via undercooked meat, contaminated soil, or by direct contact
• The antibody is specific for toxo, and can identify the trophozoites in the bloodstream, in tissue, and in reticuloendothelial cells, leukocytes and in epithelial cells

 Staining pattern

• Membranous

References:

1. Conley et al: Toxoplasma gondii infection of the central nervous system. Use of the PAP method to demonstrate Toxoplasma in formalin-fixed, paraffin-embedded tissue sections. Hum Pathol 12:690-698, 1981.