• All IHC stains will include a positive control tissue

• Myogenin has been detected in the majority of routinely processed rhabdomyosarcomas and Wilms tumors
• Myogenin is useful in a panel of immunostains (or frozen and/or paraffin sections) to evaluate small blue round cell tumors and poorly differentiated sarcomas
• No immunoreactivity has been observed in Ewing's sarcoma/pPNET or in neuroblastomas
• Myogenin will stain the nuclei of myoblasts from human fetal limbs, however no reactivity is observed in adult skeletal muscle¹
• Myogenin has not stained leiomyosarcoma, liposarcoma, alveolar soft part sarcoma, malignant fibrous histiocytoma, synovial sarcoma and malignant peripheral nerve sheath tumor in the small number of reported cases ²

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Myogenin is a member of a family of myogenic regulatory proteins which includes MyoD, myf5 and MRF4
• There genes encode transcription factors essential for muscle development
• Expression of Myogenin is restricted to cells of skeletal muscle origin
• Nuclear expression of myogenin is inversely related to the degree of cellular differentiation of rhabdomyosarcoma tumor cells¹

 Staining pattern

• Nuclear staining only
• Regional staining pattern is common

References

1. Wang NP, et. al.: Expression of Myogenic Regulatory Proteins (Myogenin and MyoD1) in Small blue Round Cell Tumors of Childhood. Amer J. Pathol., 147(6):1799-1810, 1995.
2. Tallini G, et. al.: Myogenic Regulatory Protein Expression in Adult Soft Tissue Sarcomas. Amer J. Pathol, 144(4):693-701, 1994.