• All IHC stains will include a positive control tissue

HNF-1 should be used in a panel of immunohistochemical stains to differentiate clear cell carcinomas of the ovary and endometrium from other malignancies. At this time, HNF-1 is not considered to be sensitive or specific enough to use alone ¹

• HNF-1 typically shows diffuse positivity in clear cell carcinomas of the ovary and endometrium (reported sensitivity of 82-89% and specificity of 55.9-97%)^1-3
• HNF-1 positivity has been reported in: High grade serous carcinoma, endometrioid carcinoma of the uterus and ovary, yolk sac tumor, mesonephric adenocarcinoma, and carcinomas from the lung, thyroid, pancreas, liver, gastrointestinal tract and GU tract
• HNF-1 is positive in normal liver, pancreas, kidney, upper and lower GI tract, secretory endometrium, and gestational endometrium

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Component of the hepatocyte nuclear factor family, transcription factors which regulate glucose metabolism in liver, kidney, small intestine and thymus

Staining pattern

• Nuclear staining pattern; cytoplasmic staining is non-specific

References

1. Kobel et al. A limited panel of immunomarkers can reliable distinguish between clear cell and high-grade serous carcinoma of the ovary. Am J Surg Pathol 2009;1(33):14-21.
2. Lim et al. Immunohistochemical comparison of ovarian and uterine endometrioid carcinoma, endometrioid carcinoma with clear cell change and clear cell carcinoma. Am J Surg Pathol 2015;39(8):1061-1068.
3. DeLair et al. Morphologic spectrum of immunohistochemically characterized clear cell carcinoma of the ovary: a study of 155 cases. Am J Surg Pathol 2011;35(1):36-44.