• Useful in identifying tumors of breast origin, although negative GCDFP-15 stains do not exclude possibility of breast carcinoma¹
• Approximately 77% of infiltrating ductal and 53% of invasive lobular carcinomas are GCDFP-15 positive
• Useful in discriminating primary ovarian tumors from breast cancers metastatic to ovary²
• Useful in battery with S-100, CEA, and ER in discriminating primary lung carcinomas from breast carcinoma metastatic to lung³

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• GCDFP-15 is a protein identified in gross cystic fluid of breast
• GCDFP-15 is expressed by some breast carcinomas (particularly those with apocrine differentiation), apocrine and eccrine glands, minor salivary glands, bronchial glands, and related tumors
• GCDFP-15 is expressed in virtually 100% of cases of Paget’s disease of skin (extramammary)
• GCDFP-15 expression has also been identified in prostate and rarely in ovarian, renal, and bladder carcinomas

Staining pattern

• “Mosaic”*, cytoplasmic based staining.
  * mosaic pattern refers to very focal or patchy reactivity

References

1. Wick, et. al: Gross Cystic Disease Fluid Protein – 15 as a Marker for Breast Cancer; Human Pathol 20:281-287, 1989.
2. Monteagudo, et. al: Value of Gross Cystic Disease Fluid Protein-15 in Distinguishing Metastatic Breast Carcinomas Among Poorly Differentiated Neoplasm Involving the Ovary; Human Pathol 22:368-372, 1991.
3. Raab, et. al: Adenocarcinoma in the Lung in Patients with Breast Cancer; American Journal of Clinical Pathol 100:27-35, 1993.