All IHC stains will include a positive control tissue

• CD61 is the most sensitive and most specific of the platelet glycoprotein antibodies
• CD61 is the earliest glycoprotein to be expressed during normal megakaryocyte maturation
• 80% of AML-M7 react with CD61; 20% are more immature and lack CD61 (diagnosed by the presence of platelet peroxidase by EM)
• The platelets from patients with Glanzmann's thrombasthenia lack both glycoproteins IIb and IIIa
• CD61 can identify small platelet clumps (microthrombi) and may be of use in renal transplantation biopsies as well as studies of ischemic heart disease, inflammatory/ ischemic bowel disease, and placental pathology, etc.
• No cross reactivity with a variety of formalin-fixed paraffin-embedded normal and neoplastic tissues have been reported

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CD61 is the integrin B-3-chain of the vitronectin receptor. This protein is found on platelets and megakaryocytes, and can associate with either glycoprotein IIb or vitronectin receptor ?-chain (CD51)

Staining pattern

• Cytoplasm of megakaryocytes, surface of platelets
• Well preserved in formalin
• Loss of antigenicity has been reported with mercuric fixatives

References

1. Gatter KC, et al. The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology 1988; 13:257-67.
2. Erber WN, et al. Detection of cells of megakaryocyte lineage in hematological malignancies by immuno-alkaline phosphatase labeling cell smears with a panel of monoclonal antibodies. British Journal of Hematology 1987; 65:87-94.
3. Cheson BD, et al. Report of the National Cancer Institute sponsored workshop on definitions of diagnosis and response in acute myeloid leukemia. Journal of Clinical Oncology 1990; 8(5):813-9.