Legionella species by rapid PCR-8188
Test info
Fluoribacter dumoffii
Legionella
Legionella sp
Legionella spp
Legionella bozemanae
Legionella dumoffii
Legionella gormanii
Legionella hackeliae
Legionella jordanis
Legionella longbeachae
Legionella micdadei
Legionella oakridgensis
Legionella pneumophila
Legionella wadsworthii
Legionellosis
Legionnaires disease
LGS
- Sensitive and rapid diagnosis of pneumonia caused by Legionella species
- The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment
Specimen
Bronchoalveolar lavage
Chest fluid
Chest tube drainage
Empyema
Lung fluid
Pericardial fluid
Pleural fluid
Sputum
Thoracentesis
Tracheal secretions/aspirates
Transtracheal aspirate
Sterile vial
Bronchial specimens
Refrigerated (preferred) - 7 days
Frozen - 7 days
Ambient - NO
Lung tissue
Frozen (preferred) - <7 days
- PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.
- Tissue in formalin, formaldehyde, or acetone
- Formalin-fixed paraffin-embedded (FFPE) block
Performance
Rapid Polymerase Chain Reaction (PCR)
Clinical and Interpretive info
Negative
A positive polymerase chain reaction (PCR) result for the presence of a specific sequence found within the Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to Legionella infection or environmental/water Legionella DNA in the specimen.
A negative PCR result indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of Legionella species in quantities less than the limit of detection of the assay.