Rapid diagnosis of Cryptosporidium and Giardia simultaneously for patients presenting with watery diarrhea, abdominal bloating, cramping

• A negative result does not exclude the possibility of the presence of Cryptosporidium or Giardia. Results should be interpreted by the clinician in conjunction with other clinical information.
• Negative results can occur in samples containing levels of antigen below the lower limits of detection of the assay. Multiple specimens collected over several days can be tested for patients suspected of being positive for Giardia or Cryptosporidium.
• The test is designed for use with stool samples collected in an acceptable transport media. The use of colonic washes, aspirates, or other diluted sample types has not been established and could affect the performance of the assay. Stool samples contaminated by products with an oily or particulate base (eg. Barium, mineral oil, etc.) could interfere with the test and are not recommended.

Screw cap plastic container (non sterile)

Stool specimen collection

1. Transfer stool to a C&S (Cary-Blair) preservative vial within 1 hour of collection if at ambient temperature.
•  Use the spoon in the vial and fill to the "fill line"
• Do not overfill
2. Mix well
3. Refrigerate

Stool transport media/vial - Culture and sensitivity (C&S) 

Screw cap plastic container (non sterile)

Stool transport media/vial - Culture and sensitivity (C&S) - 7 days refrigerated

Screw cap plastic container (non sterile) - <= 48 hours refrigerated

• Improper label (unlabeled or mislabeled)
• Time delay between time of collection and receipt of specimen
• Unpreserved specimen received at AHL more than 48 hrs after collection
• Improper transport container
• Stools in PVA fixative or EcoFix
• Improper transport temperature
• Inappropriate specimen type
• Leaking container
• Overfilled transport vial
• More than 1 specimen/day
• Concentrated stools/formed stools

Immunoassay

Negative

97.3% Sensitivity and 100% specificity for Cryptosporidium

100% sensitivity and 100% specificity for Giardia