Percentage and absolute counts for:

• Total T-cells (CD3+)
• B-cells (CD3-/CD19+)
• Natural killer (NK) cells (CD3-/CD56+CD16+)
• Helper/inducer (CD3+/CD4+) T-cell subset
• cytotoxic/suppressor (CD3+/CD8+) T-cell subset
• Total CD45+ lymphocyte count
• CD4:CD8 ratio

No pathologist morphology or interpretation is provided

• Immunological assessment of normal individuals and patients having, or suspected of having, immunodeficiency
• Initial screening and follow up monitoring of patients with primary immunodeficiencies
• Assessment of immune reconstitution post hematopoietic cell transplantation
• Screening for gross quantitative anomalies in lymphocyte subsets in infection or malignancies
• Any clinical scenario where enumeration of major lymphocyte subsets in peripheral blood is indicated

This test is not intended for the detection of phenotypically aberrant lymphocytes or blasts; for the detection of aberrant lymphocytes or blasts, a peripheral blood morphology should be ordered

Lavender (EDTA), 4mL

Immediately following collection, mix tube thoroughly by gentle inversion, 8 - 10 times, to prevent clotting

• Submit whole blood specimen in lavender (EDTA) tube
• Do not spin

Whole blood:

Lavender (EDTA), 4mL

Ambient (strict) - 72 hours

• Due to gradual cell degradation over time, testing within 24 hours is strongly recommended. However, specimens up to 72 hours will be accepted.

Refrigerated - NO

Frozen - NO

• Wrong container (anticoagulant or solution)
• Improper blood/anticoagulant ratio
• Delay in transport
• Improper storage
• Interfering substances
• Leaking container
• Cellular viability less than 50%
• Improper label (unlabeled or mislabeled)

BD Multitest 6-Color TBNK reagent with Trucount tubes

Appropriate age-specific reference values will be provided on the report