All IHC stains will include a positive control tissue

• Breast and skin adnexa are usually strongly EMA positive
• Variety of other carcinomas that are EMA positive include carcinomas of endometrium, kidney, thyroid, stomach, pancreas, lung, colon, ovary, prostate, cervix
• EMA positivity may be seen in mesotheliomas; transitional and squamous cell carcinomas may show variable reactivity
• EMA also labels plasma cells, NHL with plasmacytic differentiation, and anaplastic large cells lymphomas (Ki-1 lymphomas)
• Hepatocellular carcinomas, adrenocortical carcinomas, embryonal carcinomas and basal cell carcinomas are (usually) negative for EMA

EMA positivity in NHL and HD:

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• EMA is a glycoprotein isolated from human milk fat globule membranes
• EMA is present in many types of epithelia
• EMA can also be identified in mesenchymal and hematopoietic cells

Staining pattern

• Cytoplasmic based staining and +/- cell surface membrane staining