Anti-GBM antibodies (glomerular basal membrane; Goodpasture antigen) are important for the diagnosis of glomerulonephritis, which may be accompanied by pulmonary hemorrhage (Goodpasture pulmo-renal syndrome). Due to clinical symptoms similar with systemic vasculitis, it is appropriate to concurrently carry out tests for anti-GBM and Antineutrophil cytoplasmic antibodies (ANCA).

Gold serum separator (SST) tube

Immediatley following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

1. Allow sample to clot
2. Spin
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Transfer vial/tube with cap - 12mL (LabCorp)

Ambient (preferred) - 14 days

Refrigerated - 14 days

Frozen - 14 days

• Microbially contaminated, heat-treated specimens or specimens containing visible particulate
• Grossly hemolyzed specimen
• Grossly lipemic specimen

Multiplex flow immunoassay

Negative;  <1.0 units/mL

This test is often used in conjunction with the antineutrophil cytoplasmic antibody (ANCA) test for Wegener granulomatosis and vasculitis.