Determination of blast cell phenotype

Pathology orders only

Lavender (EDTA), 4mL 

Whole blood:

Lavender (EDTA), 4mL

Body fluid

• Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
  • Do not add anticoagulant or holding media
  • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Bone marrow

• Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
  • Specimens collected in EDTA are acceptable, but not preferred
• Transfer to an anticoagulant free sterile tube
• If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable

Spinal fluid

• Collect 5 mL (minimum 1 mL) in a sterile vial/container.
  • Do NOT add any anticoagulant or holding media
  • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high

Tissue

• Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Spinal fluid

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
  • Tissues submitted in saline or B+ fixative are not acceptable and will be rejcted
• Improper blood/anticoagulant ratio
• Delay in transport
• Improper storage
• Inappropriate timing of collection
• Interfering substances
• Leaking container

Flow cytometry

Tubes and Markers:

Tubes:
M1, M2, M3, M4, M5

Markers:CD2, CD3(cy), CD5, CD7, CD10, CD11b, CD13, CD14, CD15, CD16, CD19, CD22(cy), CD33, CD34, CD36, CD38, CD45, CD45RA, CD56, CD64, CD65, CD71, CD79a(cy), CD117, CD123, CD235a, CD300e, CD371, HLA-DR, MPO(cy), TdT(n)