• All IHC stains will include a positive control tissue

• Use in a panel for neuroendocrine tumors
• Other neuroendocrine markers include chromogranin, Leu-7 (and NSE)
• Synaptophysin is more specific than NSE and has not been detected in non-neuroendocrine cells
• Synaptophysin is usually used in combination with chromogranin to increase the sensitivity

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Synaptophysin is a glycoprotein present in pre-synaptic vesicles of neurons, and in neurosecretory granules of neuroendocrine cells

Staining pattern

• Diffuse granular cytoplasmic-based staining in neuroendocrine cells
• Punctate cytoplasmic-based staining in neurons