All IHC stains will include a positive control tissue

• ER is used to determine if a tumor expresses estrogen receptor (i.e. for classification purposes)
• ER positivity is used to determine if a patient would be a candidate for anti-estrogen therapy
• Note: ER positivity may be seen in 5-10% of lung, nonsmall cell carcinomas (up to 18% with our previous antibody 1D5); staining for ER in lung tumors is usually focal and variable in intensity ⁷; In the context of an ER-positive lung neoplasm, strong and extensive TTF-1 immunoreactivity can be regarded as strong supportive evidence for a primary bronchogenic adenocarcinoma

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• ER is a nuclear protein that binds estrogen hormone
• ER expression is seen in a variety of tumors from breast or gynecologic origin
• ER expression can also be seen in tumors of lung, stomach, thyroid, and very rarely colorectal origin

 
Staining pattern

• Nuclear staining

References

1. Deamant FD, et al: Estrogen receptor immunohistochemistry as a predictor of site of origin in metastatic breast cancer. Appl Immunohistochem 1(3): 188-192, 1993.
2. Esteban JM, et al: Biologic significance of quantitative estrogen receptor immunohistochemical assay by image analysis in breast cancer. Am J Clin Pathol 1994: 102:158-162.
3. Aziz DC: Quantitation of estrogen and progesterone receptors by immunocytochemical and image analyses. Am J Clin Pathol 1992; 98:105-111.
4. Wilbur DC et al: Estrogen and progesterone receptor detection in archival formalin-fixed, paraffin-embedded tissue from breast carcinoma: A comparison of immunohistochemistry with the dextran-coated charcoal assay. Mod Pathol, 5(1), 1992.
5. Esteban JM et al: Improvement of the quantification of estrogen and progesterone receptors in paraffin-embedded tumors by image analysis. Am J Clin Pathol, 1993; 99:32-38.
6. Taylor CR: Paraffin section immunocytochemistry for estrogen receptor; the time has come. Cancer 77(12), 1996.
7. Lau SK et al: Immunohistochemical expression of estrogen receptor in pulmonary adenocarcinoma. Appl Immunohistochem Mol Morphol. 2006 Mar; 14 (1): 83-7.