21-hydroxylase autoantibodies-13424
Test info
Addison's disease (chronic primary adrenal insufficiency) is commonly caused by autoimmune destruction of the adrenal cortex and is characterized by the presence of adrenal cortex autoantibodies in the serum. It can occur sporadically or in combination with other autoimmune endocrine diseases, that together comprise Type I or Type II autoimmune polyglandular syndrome. The microsomal autoantigen, steroid 21-hydroxylase enzyme has been shown to be the primary autoantigen associated with autoimmune Addison disease. Measurement of 21-hydroxylase autoantibodies aids in the diagnosis and management of patients of adrenal insufficiency.
Specimen
Submission of the minimum volume does not allow for repeating testing
Immediatley following collection, mix sample by gently inverting 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within one (1) hours of sample collection
Gold serum separator (SST) tube
- Allow sample to clot
- Spin within one (1) hours of sample collection
- Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
Ambient (preferred) - 14 days
Refrigerated - 14 days
Frozen - 2 years
Freeze/thaw cycles - stable x 6
- Gross hemolysis
- Gross lipemia
Performance
Enzyme-linked Immunosorbent Assay (ELISA)
Clinical and Interpretive info
Negative