All IHC stains will include a positive control tissue

• CD8 is used to identify cytotoxic/suppressor T cells
• This antibody is used in a panel with other T cell markers, to assess antigen expression relationships of benign and malignant T cell populations; unexpected expression and or loss of CD8 can occur in neoplasms
• CD4 is often used with CD8 to evaluate helper/suppressor relationships of T lymphocytes

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CD8 antibody reacts with the CD8 protein associated with the suppressor-cytotoxic subset of T lymphocytes
• CD8 is expressed with CD4 in thymocytes and in a very small number of circulating lymphocytes

Staining pattern

• Cell surface membrane staining

References

1. Mason DY et al: Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. J Clin Pathol 1992; 45:1084-8
2. Macon WR, et al: T-cell subset analysis of peripheral T-cell lymphomas by paraffin section immunohistology and correlation of CD4/CD8 results with flow cytometry. Am J Clin Pathol, 109:610-617, 1998