• All ISH stains will include a positive control tissue

• EBV is a member of Herpes virus family, and is the causative agent in infectious mononucleosis EBV is associated with some cases of Hodgkin's disease, Burkitt's lymphoma, peripheral T-cell lymphoma, gastric carcinoma and nasopharyngeal carcinoma EBV is also associated with post-transplant lymphoproliferative disorder (PTIPD)
• The EBV in situ test is much more sensitive than the EBV IHC test

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

In situ hybridization and microscopic examination

If requested, an interpretive report will be provided

Probe

EBV (EBER; Epstein-Barr Virus Early RNA)+R40 RNA probe

Specifications

Recognizes early RNA transcript of EBV infection The RNA transcript accumulates in nuclei of actively infected cells

Staining pattern

• Nuclear

References

1. Ambinder RF et al: Epstein-Barr-encoded RNA in situ hybridization: diagnostic applications. Human Pathol 25:602-605, 1994.

2. Randhawa P.S et al: Recurrent Epstein-Barr virus-associated lesions in organ transplant recipients. Hum Pathol. Feb;27(2):157-64. 1996.