POU2F3 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

  
POU2F3 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  

POU2F3 nuclear positivity has been associated with small cell carcinoma with low/no expression of  TTF-1 and standard neuroendocrine markers (CD56, INSM1, chromogranin, synaptophysin) .  Compared with other molecular subtypes of small cell carcinoma, the POU2F3 subtype is reported to have a higher frequency of combined histology.

Specimen

  
Tissue
  

Prepare a formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair

Performance

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info

  

If requested, an interpretive report will be provided.

  

Specifications

POU2F3 (POU class 2 homeobox 3/OCT-11/PLA-1/SKN-1) is a transcription factor of cellular differentiation of tuft cells in the skin, oropharyngeal, gastrointestinal and respiratory tract.  POU2F3 has been described as a molecular subtype of small cell carcinoma, comprising approximately 8% of small cell carcinomas.

Staining pattern Nuclear

References

  1. Baine, M. K. et al. SCLC subtypes defined by ASCL1, NEUROD1, POU2F3, and YAP1: a comprehensive immunohistochemical and histopathologic characterization. J. Thorac. Oncol. 15, 1823–1835 (2020).
  2. Huang, Y.-H. et al. POU2F3 is a master regulator of a tuft cell-like variant of small cell lung cancer. Gene Dev. 32, 915–928 (2018).
  3. Rekhtman, N. Lung neuroendocrine neoplasms: recent progress and persistent challenges. Mod Pathol 35, 36–50 (2022). https://doi.org/10.1038/s41379-021-00943-2.

Billing

  
88342 - 1st stain
88341 - each additional stain

Tracking

  
04/21/2022
  
04/25/2022