Prostaglandins are fatty acids derived from arachidonic acid metabolism. They are closely related to the Thromboxanes and Leukotrienes. Prostaglandin D2 is derived mainly from Prostaglandin H2, and is metabolized to Dihydroketo Prostaglandin D2. Prostaglandin D2 is excreted directly into the urine. The sites of highest Prostaglandin D2 activity are the brain, spinal cord, intestines, and stomach. Prostaglandin D2 is the major Prostaglandin produced by uterine tissue. Prostaglandin D2 is a potent bronchoconstrictor, neuromodulator, and anti-antithrombin agent. It also stimulates the secretion of Pancreatic Glucagon. Prostaglandin D2 has been found to have an anti-metastatic effect on many malignant tumor cells. Prostaglandin D2 production and circulating levels are drastically suppressed by aspirin and indomethacin.
Aspirin, indomethacin, or anti-inflammatory medications should be discontinued, if possible, at least 48 hours prior to collection.
Immediately following collection, thoroughly mix sample by gently inverting 5 times
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
Frozen (strict) - 60 days
Enzyme Immunoassay (EIA)/Enzyme-linked Immunosorbent Assay (ELISA)
35 - 115 pg/ml