• Total IgA
  • If Normal or Elevated: TTg IgA is performed at an additional charge
  • If TTG IgA result is Equivocal: Deamidated Gliadin IgA and Endomysial IgA will be performed at an additional charge
  • If Total IgA is low but ≥7.0: TTg IgA, TTg IgG, and Deamidated Gliadin IgA and IgG are performed at additional charge
  • If Total IgA is deficient < 7.0: TTg IgG and Deamidated Gliadin IgG are performed at additional charge

Celiac disease is characterized by gluten intolerance leading to a chronic malabsorptive disorder due to inflammation of the intestinal mucosa and flattening of the epithelium. Autoantibodies associated with celiac disease include; IgA anti-endomysial antibodies, IgG and IgA anti-gliadin antibodies and anti-reticulin antibodies. Several studies have demonstrated that IgA anti-endomysial tests have greater than 99% specificity for celiac disease with a greater sensitivity than anti-gliadin and anti-reticulin assays.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube - 5 mL

1. Allow sample to clot
2. Spin
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL), labelled as serum, within two (2) hours of sample collection

False bottom plasma/serum transport vial/tube (AHL)

Refrigerated (preferred) - 7 days

Frozen - 14 days

Ambient - NO

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
  • Heparinized plasma is not acceptable
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

Enzyme-linked immunosorbent assay (ELISA)

IgA total
Adult: 50 – 450 mg/dL 

TTg IgA
Negative: <4.0 U/mL
Weak positive: 4.0 - 10.0 U/mL
Strong positive: >10 U/mL

TTg IgG
Negative: <6 U/mL
Weak positive: 6.0 - 9.0 U/mL
Strong positive: >9 U/mL

Gliadin IgA
Negative: < 20 U/mL
Weak positive: 20 – 30 U/mL
Strong positive: >30 U/mL