• The primary use for this antibody is in distinguishing between invasive and in-situ breast carcinomas
• DCIS and infiltrating ductal carcinomas show membrane based staining. LCIS and infiltrating lobular carcinomas usually negative or show weak, non-specific cytoplasmic staining
• The clinical significance of serum cadherins levels in patients with cancer is not known, but E-Cadherin is elevated in patients with Paget's disease, metastatic melanoma, gastric and bladder cancer
• E-Cadherin is also elevated in the serum of patients with various skin disorders

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• E-Cadherin is a Ca++ dependent trans-membrane cell-cell adhesion protein, which functions as a tumor suppressor
• E-Cadherin is expressed by many different types of tumors, and can also be found in the serum in patients with various malignancies
• In breast tumors, E-Cadherin has been shown to be present in invasive ductal carcinomas of the breast, and is present in DCIS, regardless of the lesion grade
• However, E-Cadherin staining is frequently lost in invasive and in-situ lobular breast carcinomas
• This antibody has recently been used in differentiating between DCIS and LCIS

Staining pattern

• Strong, membrane based staining

References

1. Lehr HA et al: Cytokeratin 8 immunostaining pattern and E- Cadherin expression distinguish lobular from ductal breast carcinoma. Am J Clin Pathol 2000; 114:190-196.
2. Jacobs TW et al: Carcinomas in situ (CIS) of the breast with indeterminate features: Role of E-Cadherins staining in categorization. Lab Invest 80:23A, 2000.
3. Acs G et al: Differential expression of E-cadherins in ductal and lobular carcinoma of the breast. Lab Invest 80:17A, 2000.
4. Knudsen KA et al: Lack of correlation between serum levels and of E- and P-cadherins fragments and the presence of breast cancer.
5. 7.11.018. Epub 2008 May 20.