Plasma cell monitoring-12420

Test info

  
Plasma cell monitoring
  
12420
  
LAB12420
  
PATR
  
Flow
Flow cytometry
Immunophenotyping
MGUS
MM
Monoclonal gammopathy, plasma cell
Multiple Myeloma
Multiple Myeloma monitoring
P2
Plasma cell Immunophenotyping
Plasma cell follow-up
  
  • CD19
  • CD20
  • CD38
  • CD45
  • CD56
  • IRF4 (nuclear)
  • Kappa (cytoplasmic)
  • Lambda(cytoplasmic)
  • VS38(cytoplasmic)
  

Detecting residual disease in treated multiple myeloma patients at follow-up

  

The P2 assay is a high-sensitivity assay validated to a sensitivity of 0.01% (1 abnormal plasma cell/10,000 normal cells). This assay is NOT a minimal/measureable residual (MRD) assay, which requires a validated sensitivity of 0.001% (1/100,000). For follow-up of patients who are negative for residual abnormal plasma cells in the P2 assay, we recommend EDTA-anticoagulated bone marrow aspirate be submitted to the Mayo Clinic Laboratories for formal MRD testing (Mayo Clinic Laboratories test code: MRDMM).

Specimen

  
Bone marrow
  

Heparinized syringe

  
3.0 mL
  
1.0 mL
  

Collect 3.0 mL (minimum 1.0mL) bone marrow aspirate in a sodium heparinized syringe and transfer to an anticoagulant free tube.

Bone marrow specimen transport kit

  
Bone marrow core
  
  

 

  

Bone marrow aspirate:

If you do not use a heparinized syringe to collect the sample:

Dk green Sodium heparin (Na hep), no gel (preferred)

Lavender (EDTA), 4mL (acceptable)

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Bone marrow core:

RPMI with Hepes/Pen strep/FBS -15 mL

  

Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the Flow cytometry requisition

  

Ambient (preferred) - 72 hours

Refrigerated (discouraged)

Frozen - NO

  
14 days
  
  • Improper labels (unlabeled or mislabeled)
  • Improper anticoagulant or ratio
  • Delay in transport
  • Improper storage temperature affecting results
  • Improper container
  • Leaking container resulting in compromised specimen
  • Quantity not sufficient (QNS)

Performance

  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

Clinical and Interpretive info

  

Tubes:

P2

Markers:

CD19, CD20, CD38, CD45, CD56, IRF4 (nuclear), Kappa (cytoplasmic), Lambda (cytoplasmic) and VS38 (cytoplasmic).

  
The P2 assay is a high-sensitivity assay validated to a sensitivity of 0.01% (1 abnormal plasma cell/10,000 normal cells). This assay is NOT a minimal/measureable residual (MRD) assay, which requires a validated sensitivity of 0.001% (1/100,000). For follow-up of patients who are negative for residual abnormal plasma cells in the P2 assay, we recommend EDTA-anticoagulated bone marrow aspirate be submitted to the Mayo Clinic Laboratories for formal MRD testing (MCL test code: MRDMM).

Billing

  
88184
88185 × 8*
*Charges may vary based on immunophenotype of cells in the specimen
  
88184 - 17021303
88185 (each) - 17021304
  
88184 - 30881840
88185 (each) - 308818500

Tracking

  
06/03/2021
  
06/04/2021