• All IHC stains will include a positive control tissue

• Use for internal control to gauge general preservation of antigens in tissue (particularly useful in blocks processed at outside hospitals).¹
• Use to identify mesenchymal cells (lymphocytes are not consistently stained; some glial cells are positive)
• Vim may also be expressed in rapidly dividing epithelial cells
• Many carcinomas are CK and Vim positive:
  • Breast
  • Choroid plexus
  • Endometrium
  • Kidney
  • Lung
  • Ovary
  • Prostate
  • Salivary gland
  • Stomach
  • Thyroid

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

Submit a formalin-fixed, paraffin embedded tissue block

Ambient (preferred) 

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

Specifications

• Reacts with 57-KD intermediate filament vimentin, found in a variety ofmesenchymal cells including fibroblasts, endothelial cells, melanocytes, lymphoid cells, and macrophages

Staining pattern

• Cytoplasmic based staining

References

1. Battifore, H: Assessment of Antigen Damage in Immunohistochemistry: The Vimentin Internal Control; American Journal of Clinical Pathol 96:669-671, 1991.
2. Azumi, et. al.: The Distribution of Vimentin and Keratin in Epithelial and Non-epithelial Neoplasms: A Comprehensive Immunohistochemical Study on Formalin- and Alcohol-Fixed Tumors; American Journal of Clinical Pathol 88:286-296, 1987