Hemoglobin electrophoresis cascade, blood-994
Test info
Alpha Globin Variant
Alpha Thalassemia
Barts Hemoglobin
Beta Globin Variant
Beta Thalassemia
H Disease
- Hemoglobin A2
- Hemoglobin F
- Hemoglobin Electrophoresis
Diagnosis and comprehensive classification of thalassemias and hemoglobin variants
Specimen
Send specimen in original tube; do not aliquot
Two (2) Lavender (EDTA), 4mL
Sodium heparin (Na hep) whole blood
Yellow ACD-B
Dk green Sodium heparin (Na hep), no gel
Refrigerated (strict) - 7 days
Ambient - NO
Frozen - NO
Abnormals kept for 14 days
Performance
A2F: Cation Exchange/High-Performance Liquid Chromatography (HPLC)
HBEL: Capillary Electrophoresis
IEF: Isoelectric Focusing
MASS: Mass Spectrometry (MS)
HPFH: Flow Cytometry
UNHB: Isopropanol and Heat Stability
HBELA: Consultative Interpretation
Clinical and Interpretive info
Hemoglobin A
1 - 30 days: 5.9 - 77.2%
1 - 2 months: 7.9 - 92.4%
3 - 5 months: 54.7 - 97.1%
6 - 8 months: 80.0 - 98.0%
9 - 12 months: 86.2 - 98.0%
13 - 17 months: 88.8 - 98.0%
18 - 23 months: 90.4 - 98.0%
≥ 24 months: 95.8 - 98.0%
Hemoglobin A2
1 - 30 days: 0.0 - 2.1%
1 - 2 months: 0.0 - 2.6%
3 - 5 months: 1.3 - 3.1%
Hemoglobin F
1 - 30 days: 22.8 - 92.0%
1 - 2 months: 7.6 - 89.8%
3 - 5 months: 1.6 - 42.2%
6 - 8 months: 0.0 - 16.7%
9 - 12 months: 0.0 - 10.5%
13 - 17 months: 0.0 - 7.9%
18 - 23 months: 0.0 - 6.3%
≥ 24 months: 0.0 - 0.9%
≥ 6 months: 2.0 - 3.3%
Billing
83020 - Quantitation by electrophoresis
83021 - Quantitation by HPLC