Lambda light chains in situ hybridization (ISH)-12376 - Technical only, 12379 - Technical & interpretation

Test info

  
Lambda light chains in situ hybridization (ISH)
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Lambda
Lambda ISH
  
  • All ISH stains will include a positive control tissue
  

Kappa and Lambda stains are used to determine monoclonality in malignant plasmacytic or lymphoid populations, and polyclonality in benign populations

Specimen

  
Tissue
  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair

Performance

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

In-situ hybridization(ISH) and microscopic examination

Clinical and Interpretive info

  

If requested, an interpretive report will be provided

  

Specifications

Lambda antibody reacts with free kappa chains and kappa chains in immunoglobulin complexes

Staining pattern

  • Cytoplasmic staining is present in plasma cells, and in plasmacytoid lymphocytes
  • Cell surface membrane staining can be seen on B-lymphocytes (this pattern is less likely to be identified on paraffin sections, but is more easily identified on frozen tissue)
  • Since endogenous immunoglobulin is present in all human tissues, diffuse "background" staining is expected.

References

1. Wolf BC, et al: An evaluation of immunohistologic stains for immunoglobulin light chains in bone marrow biopsies in benign and malignant plasma cell proliferations. Am J Clin Pathol 94:642-746, 1990.

Billing

  
88365 - 1st stain
88364 - each additional stain

Tracking

  
07/17/2017
  
08/04/2022
  
01/12/2024