• p-ANCA
• c-ANCA
• If c-ANCA or p-ANCA Screen is positive, a titer will be performed at additional charge
• If p-ANCA, c-ANCA or Atypical ANCA are positive, testing will reflex to the following at an additional charge:
  • Myeloperoxidase Aby
  • Proteinase 3 Aby

ANCA (anti-neutrophil cytoplasmic antibodies) occur in patients with granulomatosis with polyangitis (GPA), microscopic polyarteritis, necrotizing or crescentic glomerulonephritis, other vasculitides and inflammatory bowel disorders (primarily ulcerative colitis).

Gold serum separator (SST) tube

Immediatley following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of collection

Gold serum separator (SST) tube

Red serum vial/tube - 5 mL

Red/grey serum separator (SST) vial/tube, 10 mL

1. Allow specimen to clot
2. Spin
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL) within two (2) hours of collection

False bottom plasma/serum transport vial/tube (AHL)

Red/grey serum separator (SST) vial/tube, 10 mL

Refrigerated - 7 days

Frozen (OK)

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
  • Heparinized plasma is not acceptable
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)
•  

Immunofluorescence Assay (IFA)

p-ANCA: Negative

c-ANCA: Negative

If c-ANCA Screen is positive, c-ANCA titer will be performed at an additional cost. 

Screening performed at 1:20 dilution.
Titers performed for positive c-ANCA and p-ANCA at the following dilutions: 1:40, 80, 160, 320, 640, 1280. ANA will be performed at 1:20 on any Atypical p-ANCA to rule out interference. Note that ANA at this titer is utilized in the laboratory for interpretation only. It is not a reportable ANA result and should not be interpreted as a positive ANA for the diagnosis of lupus or other auto-immune disease.

Positive or atypical c-ANCA and p-ANCA will reflex to Myeloperoxidase Antibodies and Proteinase 3 Antibodies, IgG at an additional charge.