p53 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

p53 by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue

P53 over-expression is present in many different malignancies, such as colon, stomach, bladder, breast, lung, testes, melanomas, sarcomas, etc.




Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • P53 is a transcription factor that regulates cell growth and inhibits cells from entering S-phase
  • P53 is a DNA-binding phosphoprotein coded for by a tumor suppressor gene located on the short arm of chromosome 17
  • Mutations of p53 or functional inactivation with intact p53 genes are common in many cancers, leading to loss of normal growth regulation
  • Although p53 is present in small amounts in normal cells (not usually detected by routine immunohistochemistry), mutations resulting in a prolonged half-lives lead to accumulations that are detected by immunohistochemistry
  • Over-expression is associated with a poor prognosis in many cancers

Staining pattern

  • Nuclear staining


  1. Bartek J et al: Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene 1991; 6: 1699-703.


88342 - 1st stain
88341 - each additional stain