Identification, susceptibilities and serogrouping will be performed on all microbiology isolates considered by the laboratory to be significant.
Anaerobes are part of the endogenous flora of the body, so it is important to culture only specimen from sites that do not have normal anaerobic flora (refer to reasons for rejection).
Sources appropriate for anaerobic culture include: Abscess aspirate (including rectal, oral and vaginal), body fluid, bronchoscopy, Cerebral spinal fluid (CSF), IUD for Actinomyces species, placental tissue from C-section delivery, sinus aspirate/swab, surgical specimens not contaminated with bowel flora, tissue, transtracheal aspirate or urine collected by suprapubic aspirate.
Specimen MUST be in ESwab or anaerobic transport media.
Allina Health metro hospital collections may be sent directly to the lab in a capped syringe (no needles) with air expressed out within one hour of collection.
ESwab transport tube
A.C.T. culture transport
ESwab transport tube in the liquid without the swab
ESwab transport tube (blue or green)
ESwab transport tube (blue or green) in the liquid without the swab
A.C.T. I culture transport tube:
Ambient - 48 hours
Ambient (preferred) - 48 hours
Refrigerated - 48 hours
Anaerobic organisms are involved in a variety of infections. Specimens from appropriate sites are cultured to isolate potential pathogens. Anaerobes are part of the endogenous flora of the body, thus it is important to culture only specimens from sites that do not have normal anaerobic flora. When possible, tissue specimens or specimens collected by needle and syringe are preferred.
Most anaerobic infections are polymicrobial and involve anaerobic, aerobic and facultative anaerobic organisms.
The identification of anaerobic organisms is based on, colony morphology, gram stain morphology, growth in the presence of bile, susceptibility to antibiotics, biochemical tests and other available testing methods.