Tropheryma whipplei, molecular detection, PCR, varies-994

Test info

Tropheryma whipplei, molecular detection, PCR, varies
Whipple's Disease

Aids in the diagnosis of Whipple disease, especially for identifying inconclusive or suspicious cases


Synovial fluid
0.5 mL
  • Container/tube:
    • Preferred: Lavender top (EDTA)
    • Acceptable: Navy (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container
  1. Place a minimum of 0.5 mL of fluid into a Lavender (EDTA) tube 
  2. Mix by gentle inversion
  3. Send specimen in original tube (preferred)
Fresh tissue or biopsy
Minimum 5 mm(3)

Fresh tissue or biopsy

  • Sources: Small intestine tissue (duodenum, ileum, or jejunum), lymph node, other visceral tissue, synovial tissue, gastrointestinal tissue, heart valve, or brain
  • Specimen volume: Entire collection or 5 mm3
  1. Collect fresh tissue specimen
  2. Submit tissue only, do not add fluid to tissue


  • Sources: Cerebrospinal or ocular (eg, vitreous humor)
  • Specimen volume: 0.5 mL

Sterile vial/container


Refrigerated (preferred): <7 days

Frozen: <7 days

1 week
  • Slides
  • Bone marrow
  • PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.


Mayo Clinic Laboratories (TWRP): R-NX
Mo - Fr
2 - 7 days

Real-time Polymerase Chain Reaction (PCR)

Clinical and Interpretive info

  • A positive result indicates the presence of Tropheryma whipplei DNA.
  • A negative result indicates the absence of detectable T whipplei DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of T whipplei DNA in quantities less than the limit of detection of the assay.