All IHC stains will include a positive control tissue.

• This marker may be used to identify sebaceous neoplasms, including sebaceous carcinoma and sebaceoma (sensitivity is approximately 97%)
• ADP will also stain milk fat globule membranes and lipid droplets, such as in adrenal gland (zona fasciculata), testes (Sertoli cells) and in lactating breast
• Staining for ADP has been reported in eccrine apocrine carcinomas, xanthomatous lesions, and metastatic renal cell carcinomas (but the pattern is often granular which is considered negative for ADP

Immunostains in evaluation of possible sebaceous tumors

Submit a formalin-fixed, paraffin embedded tissue block

FFPE tissue block

Tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Adipophilin (ADP) is a protein present on the surface of intracellular lipid droplets
• ADP is present in sebaceous lesions (particularly well-differentiated tumors)

Staining patterns

• Cytoplasmic membranous pattern of intracytoplasmic lipid globules
• Granular staining is considered negative for ADP

References

1. Ferringer T. Immunohistochemistry in dermatopathology. Arch Pathol Lab Med. 2015;139:83-105.
2. Ansai S et al: Sebaceous carcinoma: an immunohistochemical reappraisal. Am J Dermatopathol. 2011;33(6)579-587.